In vitro and in vivo microbiological evaluation of six mouthwashes manufactured in Egypt

Six antiseptic mouthwash, manufactured in Egypt, were microbiologically evaluated by in vitro and in vivo techniques. The level and type of microbial contaminants were determined. The mouthwash clinitol and citrolin were significantly contaminated, while Antiseptol, Betadine, Hexitol and Oraldene were not. Tube dilution and sub-culturing on solid media were used to assess the antimicrobial efficacies of the mouthwash against 24 clinical strains also kill times were determined for undiluted and half- concentrated mouthwash in presence and absence of human blood and saliva. Generally, the results varied not only between test strains but also mouthwash including those containing identical active principles thus implicating formulation factors